6 In 1969, fluorescence activated cell sorting (FACS) was born. “[Leonard Herzenberg] had the concept of FACS and developing it further. And that was not something I would have done,” Leonore ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

cell cycle analysis, kinetics, proliferation, calibration and statistical comparisons. Other examples include BD CellQuest, a popular commercial flow cytometry data acquisition and analysis ...

Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...

flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental to investigations of cell cycle–regulated protein expression ...

For almost six decades, flow cytometry (FC) has been a cornerstone of clinical and laboratory research, with applications in a wide range of fields, including immune-phenotyping, diagnostics, cell ...

Cell cycle and apoptosis were determined by flow cytometry. The messenger RNA and protein levels of target genes were analyzed using quantitative real-time PCR and western blot. According to the ...

One important aspect of DNA analysis is the ability of the cytometer to exclude cell doublets. In a cytometer where the beam diameter is larger than the nuclear size, we can look at pulse width versus ...