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The trypsin-EDTA (Sigma) was inactivated using two volumes of complete growth medium. Prior to seeding the gut-on-chips with HCT-8 cells, the microchannels were rinsed with 70% (v/v) ethanol ...

The epidermis was then gently peeled from the dermis and incubated with Trypsin-EDTA (Sigma-Aldrich, United States) on a tube revolver at 37°C for 30 min. Trypsin was deactivated by media containing ...

Medium was changed daily and cells were passaged every 2–3 days with 1× Trypsin-EDTA solution. Spinal motor neurons (MNs) were differentiated from mESCs HB9::GFP according to Wichterle et al (2002) ...

The Sigma BF draws attention with its modern industrial design and woos creative photographers with a streamlined interface and gorgeous color profiles, but it offers fewer features than ...

After that, 2 - 3 mL of 0.25% Trypsin-EDTA solution in Hanks balanced salt solution without calcium or magnesium was introduced, after which the cells were allowed to detach/dissociate from their ...

Cells at 70–80% confluency were harvested from cell culture dishes by trypsin–EDTA (Sigma-Aldrich) treatment. The harvested cells were suspended in saline and then large cell aggregates were removed ...

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Thanks for creating EDTA, keep doing the great work. I wanted to share some observations from my analysis. I have been running EDTA on several red raspberry assemblies and have noticed an unusually ...